Abstract
SummaryPotent antiserum was prepared against a murine leukemia virus (Rauscher) in BALB/c mice by an immunizing regimen consisting of two inoculations in which the second one is given only after an interval sufficient for the primary response to wane. An interval of 90 days or more following a primary injection of antigen emulsified in Freund's adjuvant was optimal. The early antibody response to Rauscher virus in ET-treated mice is also described. The gamma-M antibodies, which appeared as early as the third day after a single antigenic stimulus, were readily detected by HA tests, but failed to precipitate with virus in immunodiffusion tests.
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