Enhanced photodegradation of antibiotics based on anoxygenic photosynthetic bacteria and bacterial metabolites: A sustainably green strategy for the removal of high-risk organics from secondary effluent

Abstract

Antibiotic residues in effluents discharged from wastewater treatment plants (WWTPs) have been considered high-risk organics due to biorefractory property and potential toxicity. Secondary pollution and unsustainability existed in advanced treatment of secondary effluent are currently in urgent need of improvement. In this study, a sustainably green strategy based on Rhodopseudomonas palustris (R.palustris) by regulating the structure of extracellular polymeric substances (EPS) was proposed for the first time to achieve efficiently removal of sulfadiazine (SDZ). Results showed that 0.2 V was the optimal external potential for R.palustris to efficiently remove SDZ, where the biodegradation rate constant obtained at this potential was 4.87-folds higher than that in open-circuit mode and a complete removal was achieved within 58 h in the presence of EPS extracted at this potential. Three-dimensional excitation-emission matrix (3D-EEM) spectra analysis suggested that tryptophan protein-like, tyrosine protein-like, humic acid-like and fulvic acid-like substances present in EPS were the main effective components which was responsible for the indirect photodegradation of SDZ. The quenching experiments showed that 3EPS* was the dominant reactive species which accounted for 90% of SDZ removal. This study provides new implications for the advanced treatment of secondary effluent organic matters by developing eco-friendly bioaugmentation technology and biomaterials.