Abstract
A simple photocatalytic hydrogen-evolving system is reported based on intermolecular electron transfer using isolated Photosystem I (PSI) reaction centers as the photoactive element. The system is composed of platinized PSI covalently linked to plastocyanin (PC). Water-soluble sodium ascorbate is the electron donor. PC was attached to PSI by formation of peptide bonds with the cross-linking reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Compared to the unlinked proteins, cross-linking of PC and PSI resulted in a substantial increase in light-driven reduction of hexachloroplatinate ions (PtCl62- + 4e- → Pt↓ + 6Cl-). Hydrogen photoevolution by cross-linked PC−platinized PSI was increased 3-fold both in initial rate and total yield. Analysis of the reaction indicates that covalent linkage of PC to PSI results in a greater rate of total electron throughput from sodium ascorbate to light-activated hydrogen evolution. In addition, although photocatalytic hydrogen-evolving activity was eas...
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