Abstract
Cell suspension-derived protoplasts were cultured in (A) liquid medium, (B) medium overlaying oxygenated perfluoro-decalin (PFC), (C) medium containing 1:50 (v:v) of haemoglobin solution ( Erythrogen TM ), or (D) medium with 1:50 (v:v) Erythrogen TM overlaying oxygenated PFC. In Passiflora, mitotic division of protoplasts was increased (P < 0.05) by all treatments, with Erythrogen TM being the most effective (120% increase over control). For Petunia, treatment D induced maximum mitosis (140% over control), whilst Erythrogen TM alone produced a less pronounced (80% over control) increase.
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