Abstract
Present luminescence assays for horseradish peroxidase (HRP) have limitations. Here we report a novel procedure in which the HRP-catalysed luminescence of a cyclic hydrazide (such as luminol) is multiplied severalfold by the addition of a synthetic component of the firefly bioluminescent system, D-luciferin (4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazole-carboxylic acid). The specific enhancement of HRP-catalysed light emission from cyclic hydrazides should extend the sensitivities of luminescently monitored assays, which have already been shown to be as sensitive as those using radioactive labels1,2. This procedure has been applied to the immunoassay of serum α-fetoprotein, thyroxine, digoxin, hepatitis B surface antigen, immunoglobulin E and rubella virus antibody.
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