Abstract
The green algae, Chlamydomonas reinhardtii, is one of the model species used to study lipid production, although research has focused on nitrogen-deficient cultures, that inhibit the development of biomass by C. reinhardtii and limit lipid production. In this study, Azotobacter chroococcum was added to the algal culture to improve lipid accumulation and productivity of C. reinhardtii. The maximum lipid content and production of C. reinhardtii in the co-culture were 65.85% and 387.76 mg/L, respectively, which were 2.3 and 5.9 times the control's levels of 29.11% and 65.99 mg/L, respectively. The maximum lipid productivity of C. reinhardtii in the co-culture was 141.86 mg/(L·day), which was 19.4 times the control's levels of 7.33 mg/(L·day). These increases were attributed to the enhanced growth and biomass and the change in the activity of enzymes related to lipid regulation (ACCase, DGAT, and PDAT). Compared to the conventional strategy of nitrogen deprivation, A. chroococcum added to the culture of C. reinhardtii resulted in higher lipid accumulation and activity, greater efficiency in the conversion of proteins to lipids, higher biomass, and increased growth of C. reinhardtii. Therefore, using A. chroococcum to improve the growth and biomass of C. reinhardtii is an efficient, rapid, and economically viable strategy for enhancing lipid production in C. reinhardtii.
Highlights
The global supply of traditional fossil fuels is limited and the combustion of fossil fuels produces CO2 and other greenhouse gases that cause climate change (Hui et al, 2016)
After A. chroococcum was added to the algal culture, the maximum lipid production of C. reinhardtii in the co-culture
Growth and Biomass of Algae Co-cultured With A. chroococcum
Summary
The global supply of traditional fossil fuels is limited and the combustion of fossil fuels produces CO2 and other greenhouse gases that cause climate change (Hui et al, 2016). Under certain stress conditions, such as high light intensity or nutrient deficiency, some algae can accumulate large amounts of lipids, such as triacylglycerides. Because of their fast growth, high lipid content, Improvement of Lipid Production in Chlamydomonas reinhardtii and optimized lipid composition, microalgae are ideal materials for biodiesel production (Hu et al, 2008; Wang et al, 2009; Siaut et al, 2011). Chlamydomonas reinhardtii (C. reinhardtii) is a unicellular green algae species, whose genome has been fully sequenced It grows quickly, costs little to cultivate, and can produce lipids under nitrogen-deficient conditions; it has been widely used for lipid production (Park et al, 2015). In order to improve the lipid accumulation by C. reinhardtii, we need to identify an effective way to increase the biomass of C. reinhardtii in the absence of nitrogen
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