Abstract
A newly prepared [(ppy)2 Ir(dcbpy)](+) ⋅PF6 (-) (ppy: 2-phenylpyridyl; dcbpy: 4,4'-dicarboxy-2,2'-bipyridyl) and gold nanoparticle functionalized mesoporous silica nanoparticle (Au/Ir-MSN) is reported. Based on the binding between concanavalin A (Con A) and mannose, the novel nanoparticle was applied to an ultrasensitive electrochemiluminescence (ECL) in situ cytosensing strategy and the dynamic evaluation of cell-surface carbohydrate expression. The ECL activity of the presented Con A@Au/Ir-MSN nanoprobe was greatly enhanced by employing a functionalized nanoparticle and graphene nanomaterial with an increased surface area and simultaneously improved electron-transfer efficiency at the electrode interface. Under optimal conditions, the sandwich-type ECL cytosensor showed a linear response to K562 cells at concentrations ranging from 1.0×10(2) to 1.0×10(6) cells mL(-1) and realized a low detection limit of a single cell. The proposed method could also be successfully used for monitoring the dynamic variation of carbohydrate expression in cancer cells in response to external stimulation by an inhibitor.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
