Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs.

M Dilipkumar,M Rajasimman,N Rajamohan

doi:10.1007/s13205-012-0112-2

M Dilipkumar, M Rajasimman + Show 1 more

Open Access

https://doi.org/10.1007/s13205-012-0112-2

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Journal: 3 Biotech	Publication Date: Jan 3, 2013
Citations: 29	License type: CC BY 2.0

Affiliation: Annamalai University, Sohar University

Abstract

In this work, inulinase was produced by solid state fermentation by Streptomyces sp. using copra waste as carbon source. The nutrients were screened by Plackett–Burman design. From the pareto chart it was found that the nutrients, namely, soya bean cake, MgSO4·7H2O and (NH4)2SO4 were found to be most significant nutrient components. Hence, these three components were selected for further optimization using central composite design (CCD) in response surface methodology (RSM). The optimum conditions were soya bean cake: 0.05711 g/gds, MgSO4·7H2O: 0.00063 g/gds and (NH4)2SO4: 0.00772 g/gds. Under these optimized conditions, the production of inulinase was found to be 131 U/gds. The constants in the Michaelis–Menten equation were evaluated and high R2 value implies the fitness of the model.

Highlights

IntroductionInulin (a polyfructan consisting of linear b-2,l linked fructose) is a linear biopolymer made up of fructose residues linked by b-2,1 bonds (Vandamme and Derycke 1983) that constitute the storage of carbohydrate in the
Inulin is a linear biopolymer made up of fructose residues linked by b-2,1 bonds (Vandamme and Derycke 1983) that constitute the storage of carbohydrate in the
The R2 value was 0.9245, which was greater than 0.9 shows the fitness of the model for inulinase production

Summary

Introduction

Inulin (a polyfructan consisting of linear b-2,l linked fructose) is a linear biopolymer made up of fructose residues linked by b-2,1 bonds (Vandamme and Derycke 1983) that constitute the storage of carbohydrate in the. Inulinase is highly prevalent in higher plants and microorganisms. This enzyme can be used for isolation of fructose from raw plants: Jerusalem artichoke (Helianthus tuberosus), dahlia (dahlia), elecampane (Inula helenium), chicory (Cichorium), dandelion (Taraxacum) and burdock (Arctia) (Kovaleva et al 2009). Using copra waste as substrate was carried out using a sequential strategy of the experimental design. Copra waste is a cheap substrate which is abundantly available in southern part of India (Kerala and Tamilnadu). It was selected as substrate for the production of inulinase

Materials and methods

Results and discussion

Conclusions