Abstract
The changing concentrations of circulating protein biomarkers have been correlated with a variety of diseases. Quantitative bioassays capable of sensitive and specific determination of protein biomarkers at low levels can be essential for therapeutic treatments that can improve outcomes for patients. Herein, we describe the investigation of a rotating paper device (RPD) for quantitative determination of targeted proteins at the fM concentration level. The RPD consists of two circular papers each separately supported with a plastic disc. Protein detection is conducted via enhanced immunoassay using amplification in a sequential workflow, which includes a sandwich immunoassay in the upper paper and a signal amplification reaction in the lower paper. The sandwich immunoassay is conducted using biobarcode nanoparticles (BNPs) and results in the release of reporter oligonucleotides from BNPs. These oligonucleotides are transferred to the bottom paper, where they engage in a target recycling methodology that leads to the production of a colorimetric signal. The assay was evaluated for quantitation of interleukin-6 (IL-6), a cytokine biomarker in serum. A limit of detection of 63 fM and a dynamic range of 200 fM-8 pM was observed for the assay. The specificity of the assay was successfully verified against several common protein biomarkers.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
