Abstract
In this study, a biological thermal-alkaline synergistic system was successfully established to enhance the hydrolysis-acidification efficiency of high-solids and low-organic-content sludge (HS-LOC-S). The results indicated that the highest hydrolysis rate was obtained at pH of 12 (52.62%) leading to the highest production of soluble chemical oxygen demand (SCOD) and soluble protein (SP). The highest acidification rate was observed at pH of 10 (32.15%), leading to the highest production of volatile fatty acids (VFAs). At pH of 10, average sludge size reduced by 24.60%, and the proportion of biodegradable dissolved organic matter (DOM) produced by synergistic system increased by 15.82%, when compared with those of raw sludge. Moreover, results of 16S rRNA clearly validated that the relative abundance of hydrolytic and acidogenic microbes (e.g. Tepidimicrobium, Coprothermobacter) abundantly enriched at pH of 10 (49.88%) was greatly higher than others, which was the main reason for its maximum VFAs accumulation.
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