Abstract
Objective: We have successfully used mouse secondary polar bodies (2PBs) and female pronuclei (FPNs) to reconstruct viable embryos. However, factors attributing to the success of reconstruction remain unclear. The EGFP gene encoding a protein emitting green fluorescent light which can be easily observed by a fluorescent microscopy. In this study, we tried to established the method of integrating this exogenous DNA into the genome of the reconstructed embryos as a non-toxic biological marker to assess the efficiency of transgenesis and nuclear transplantation.
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