Abstract
ObjectiveThe objective of the present study was to evaluate the effect of umbelliferon-α-D-glucopyranosyl-(2I → 1II)-α-D-glucopyranoside (UFD) from Aegle marmelos Corr. on serum glucose, lipid profile and free radical scavenging activity in normal and STZ (streptozotocin) induced diabetic rats.Materials and methodsDiabetes was induced by single interperitoneal injecting of streptozotocin (60 mg/kg, i.p.) in the rats. All the rats were divided into following groups; I - nondiabeteic, II - nondiabetic + UFD (40 mg/kg, p.o.), III - diabetic control, IV - UFD (10 mg/kg, p.o.), V - UFD (20 mg/kg, p.o.), VI - UFD (40 mg/kg) and VII - glibenclamide (10 mg/kg, p.o.). Serum glucose level and body weight were determined periodically. Biochemical parameter, antioxidant enzyme and histopathology study were performed on the day 28. Oral glucose tolerance test study was performed to identify the glucose utilization capacity.ResultsAll the doses of UFD and glibenclamide decrease the level of serum glucose, glycated hemoglobin, glucose-6-phosphatase, fructose-1-6-biphosphate and increased the level of plasma insulin, hexokinase. The UFD doses also showed effects on antioxidant enzymes viz. superoxide dismutase, catalase and glutathione peroxidase which were significantly increased and the level of malonaldehyde was markedly decreased. Histologically study, focal necrosis, deposition of fats, increased the size of the intercalated disc were observed in the diabetic rat liver, kidney, heart and pancreas but was less obvious in treated groups. The mechanism of action of the UFD emerges to be due to increase the activity of antioxidant enzyme and secretion of pancreatic insulin.ConclusionReduction in the FBG (fasting blood glucose), glycated hemoglobin, glucose-6-phosphatase, fructose-1-6-biphosphate, superoxide dismutase, catalase, glutathione peroxides, cholesterol, triglyceride, LDL, VLDL levels and improvement in the level of the plasma insulin, hexokinase, HDL was observed by the UFD treated rats. The result indicates that UFD has anti-diabetic activity along with anti hyperlipidemic and antioxidant efficacy and provides a scientific rationale to be used as an Anti-diabetic agent.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-639) contains supplementary material, which is available to authorized users.
Highlights
Diabetes mellitus (DM) is a group of syndrome characterized by dietary intake, changing in the lifestyle, excessive use of lipid, carbohydrate and protein
All the doses of umbelliferon-α-D-glucopyranosyl(2I → 1II)-α-D-glucopyranoside (UFD) and glibenclamide decrease the level of serum glucose, glycated hemoglobin, glucose-6-phosphatase, fructose-1-6-biphosphate and increased the level of plasma insulin, hexokinase
Reduction in the FBG, glycated hemoglobin, glucose-6-phosphatase, fructose-1-6-biphosphate, superoxide dismutase, catalase, glutathione peroxides, cholesterol, triglyceride, LDL, VLDL levels and improvement in the level of the plasma insulin, hexokinase, HDL was observed by the UFD treated rats
Summary
Diabetes mellitus (DM) is a group of syndrome characterized by dietary intake, changing in the lifestyle, excessive use of lipid, carbohydrate and protein. Several species of plants have been reported in the reputed alternative system of medicine as best choice for the treatment of diabetes because plant based antidiabetic drug are considered less toxic and free from side effects. The major drawback of the natural therapy is limitation of bioactive compound for claiming their antidiabetic effect (Morin 1987). Most of the researchers claimed that diabetes complications were occurred by oxidative stress (Halliwell and Gutteridge 1989). Clinical and experimental condition of diabetes increasing the level of oxidative stress otherwise changes in antioxidant capacity and produced the etiology of chronic diabetes (Ravi et al 2004). Many investigator claim that several phenolic coumarins might play a role as dietary antioxidants, because several fruit and vegetable were consumed by human beings as food
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