Abstract
Recent reports have shown enhanced fluorescence for fluorophores in close proximity to chemically deposited silver islands or colloids. To expand the usefulness of metal-enhanced fluorescence we tested fractal silver structures formed on, or near, silver electrodes by passage of electric currents. The emission intensity of fluorescein-labeled human serum albumin (FITC-HSA) was enhanced over 100-fold when adsorbed to the fractal silver structures as compared to glass. The amplitude-weighted lifetime is dramatically reduced to near 3 ps. Enhanced fluorescence was shown to result in selective observation of FITC-HSA over a fluorophore not attached to the silver surface. And finally, photostability measurements indicate 160-fold more photons are detectable from FITC-HSA on the fractal silver surface. These results suggest the use of in situ generated silver structures for metal-enhanced fluorescence.
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