Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences.

Luigia Fonticoli,Ylenia Della Rocca,Francesca Diomede,Guya Diletta Marconi,Oriana Trubiani,Jacopo Pizzicannella,Thangavelu Soundara Rajan,Stefano Oliva,Giovanna Murmura

doi:10.3390/biomedicines9111710

Abstract

The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.

Highlights

Mesenchymal Stem Cells (MSCs) are well recognized in the regenerative and tissue engineering field for the long-lasting proliferation ability, the capability to differentiate in different lineages, immunomodulation feature and the easy accessibility [1,2,3]
The human periodontal ligament stem cells (PDLSCs) (hPDLSCs) were negative for the subsequent markers CD14, CD34 (Figure 1A)
To evaluate the morphological features of hPDLSCs the primary cultures were observed by means of inverted light microscopy

Summary

Introduction

Mesenchymal Stem Cells (MSCs) are well recognized in the regenerative and tissue engineering field for the long-lasting proliferation ability, the capability to differentiate in different lineages, immunomodulation feature and the easy accessibility [1,2,3]. Due to their valuables characteristics and their good ability in tissue and organ regeneration, they have been utilized for clinical studies [4]. The human PDLSCs (hPDLSCs) are derived from periodontal ligament, which is characterized by the presence of different cell populations, such as osteoblasts, fibroblasts, epithelial cells, endothelial cells and stem cells [7]. They are considered as a valuable stem cell population for regenerative therapy in periodontium, and they are the widely used MSCs population in the dentistry field [8]

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