Abstract
BackgroundAs a new type of molecular marker, microRNAs (miRNAs) can be used for early diagnosis and prognosis prediction of malignant tumors, and has broad clinical application prospects. This paper mainly studies the important role of miR-889 in the occurrence and development of hepatocellular carcinoma and the prognostic significance of miR-889 in hepatocellular carcinoma.MethodsQuantitative real-time PCR analysis detected the expression levels of miR-889 in hepatocellular carcinoma tissues and cell lines. Kaplan-Meier curve and Cox regression analysis were used to explore the prognostic significance of miR-889 in hepatocellular carcinoma. The CCK-8 and Transwell assays assay were used to assess cell proliferation, migration, and invasion abilities ability.ResultsThe expression of miR-889 in hepatocellular carcinoma tissues was significantly higher than that in adjacent tissues. Overexpression of miR-889 was significantly associated with TNM stage, hepatitis B virus infection, and cirrhosis. Patients with high miR-889 expression had shorter overall survival than those with low miR-889 expression. And functional studies in two hepatocellular carcinoma cell lines have shown that overexpression of miR-889 significantly promoted cell proliferation, migration, and invasion in vitro.ConclusionsOverall, miR-889 was upregulated in hepatocellular carcinoma tissues and cell lines, and overexpression of miR-889 promoted cell proliferation, migration, and invasion in hepatocellular carcinoma cells. Based on our findings, high expression of miR-889 may promote the progression of hepatocellular carcinoma, and high expression of miR-889 is also forecasted for an unfavorable prognosis in hepatocellular carcinoma.
Highlights
Hepatocellular carcinoma (HCC) is the most common primary liver tumor, and its incidence ranks fifth among malignant tumors and third among deaths caused by tumors [1, 2]
Expression of miR-889 in HCC tissues and cell lines The expression of miR-889 in HCC tissues was detected by quantitative real-time PCR (qRT-PCR)
The results showed that the expression of miR-889 in HCC tissues was significantly higher than that in adjacent tissues (P < 0.001, Fig. 1 A)
Summary
Hepatocellular carcinoma (HCC) is the most common primary liver tumor, and its incidence ranks fifth among malignant tumors and third among deaths caused by tumors [1, 2]. The occurrence of liver cancer is closely related to chronic inflammation of the liver. The occurrence and development of liver cancer are closely related to the repeated damage and proliferation of liver cells caused by chronic inflammation of the liver [5]. The molecular mechanisms mainly include the activation of oncogenes in liver cells, the inactivation of tumor suppressor genes, and the excessive activation of signal pathways related to liver cancer [6, 7]. The molecular mechanism research on the occurrence and development of liver cancer and the translational medicine research closely integrated with the clinic will propose new intervention directions for the prevention, control, and treatment of liver cancer [8, 9]. This paper mainly studies the important role of miR-889 in the occurrence and development of hepatocellular carcinoma and the prognostic significance of miR-889 in hepatocellular carcinoma
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