Enhanced Erinacine A Production by Hericium erinaceus Using Solid-State Cultivation

Po-Yu Cheng,Hung-Yi Liao,Yung-Chuan Liu,Chia-Hung Kuo

doi:10.3390/fermentation7030182

Po-Yu Cheng, Hung-Yi Liao + Show 2 more

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https://doi.org/10.3390/fermentation7030182

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Abstract

Hericium erinaceus (HE) is a large edible medicinal fungus. Erinacine A (ErA) is a secondary metabolite presented in the mycelia of HE, with pharmacological effects as a nerve growth factor on the central nervous system. In this study, solid-state cultivation of HE was carried out in Petri dishes and glass jars for the production of mycelial biomass and ErA. The potato dextrose agar (PDA) had the highest mycelial biomass at an optimal temperature of 25 °C, but no ErA was found in the agar media. In glass jar cultivation, the mycelial biomass and specific yield of ErA in different substrates, particle sizes, substrate weights, nitrogen sources, and inorganic salts were investigated. The ErA was purified by a self-pack silica gel column and a semi-preparative HPLC and was identified by liquid chromatography-tandem mass spectrometer. The best conditions for solid-state cultivation of HE when using corn kernel as substrate, particle size less than 2.38 mm, and addition of 10mM ZnSO4, 7H2O, mycelial biomass of 50.24 mg cell dry weight/g substrate was obtained, in addition, the specific yield of ErA could reach 165.36 mg/g cell dry weight.

Highlights

IntroductionMany studies have found that the fruit bodies and mycelia of Hericium erinaceus (HE) have many bioactive compounds, such as polysaccharides [3,4], hericenones [5], erinacines [6], and ergothioneine [7]
potato dextrose agar (PDA), malt extract agar (MA), and yeast extract agar (YE), were prepared for HS mycelial growth in the Petri dish plate in order to observe the growth of mycelium and the optimal culture temperature
The results indicated that the appropriate addition of NaCl or ZnSO4·7H2O could help increase the production of mycelial biomass or Erinacine A (ErA)

Summary

Introduction

Many studies have found that the fruit bodies and mycelia of HE have many bioactive compounds, such as polysaccharides [3,4], hericenones [5], erinacines [6], and ergothioneine [7]. These bioactive compounds have been reported to be helpful for human health against various physiological system diseases, including the nervous system, digestive system, circulatory system, and immune system [1,8,9,10]

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