Abstract
The results of enzymolysis studies on cotton fibres and cotton plant stalks using Penicillium funiculosum Thom F 4 are reported. The substrates were used in the never-dried state as well as in the dried condition. In addition, the effect of using substrates which were not dried subsequent to alkali pretreatment and aqueous removal of alkali thereafter was also investigated. Higher saccharification efficiencies were observed when the substrates used were kept in a wet condition prior to reaction with the enzyme. Investigations on the changes in the three-dimensional structural order in the substrates as well as the changes in the degree of polymerization during the course of enzymolysis, were helpful in understanding the differences in the results obtained with the various substrates.
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