Abstract
ABSTRACT Natural defense systems of plants can be induced with the use of elicitors. Pea (Pisum sativum L.) plants treated with different concentrations of salicylic acid and 4-aminobutyric acid increased activities of phenol metabolizing enzymes implicated in the defense of plants. The enzymes peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and superoxide dismutase responded to treatment with variation in their activities. Phenolic content also varied following treatment with the inducers. Treatment with 5 mM salicylic acid resulted in the increase in the number of peroxidase isozymes in the intercellular fluid of pea leaves. SDS-PAGE of the intercellular fluid of 5 mM salicylic acid treated pea leaves showed one new polypeptide with a MW of 96.7 kDa. Exogenous application of elicitors, particularly salicylic acid, may be employed against plant pathogens.
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