Abstract

Biological treatment of sulfate-laden wastewater consists of two separate reactors to reduce sulfate to sulfide by sulfate-reducing bacteria (SRB) and to oxidize sulfide to sulfur (S0) by sulfide oxidation bacteria (SOB). To have SRB+SOB in a single reactor faced difficulty of low S0 conversion. This study for the first time revealed that dissolved oxygen (DO) level can be used to manipulate SRB+SOB reactions in a single reactor. This work demonstrated successful operation of an integrated SRB+SOB reactor under micro-aerobic condition. At DO=0.10–0.12mgl−1, since the activities of SOB were enhanced by limited oxygen, the removal efficiency for sulfate reached 81.5% and the recovery of S0 peaked at 71.8%, higher than those reported in literature. At increased DO, chemical oxidation of sulfide with molecular oxygen competed with SOB so conversion of S0 started to decline. At DO>0.30mgl−1 activities of SRB were inhibited, leading to failure of the SRB+SOB reactor.

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