Abstract
Contrast-enhanced μCT imaging has been used to provide non-destructive 3D images of microdamage, but at a lower quality than found in histology and 2D backscatter electron (BSE) imaging. This study aimed to quantify potential improvements in microdamage characterization by enhancing µCT scanning parameters. Eleven slides from 9 rat vertebrae (healthy = 3, osteolytic metastases = 3, mixed metastases = 3) previously stained for microdamage with BaSO4 and analyzed with BSE imaging (2μm voxel spacing) were used in this study. μCT imaging conducted under varying protocols (x-ray voltage, tube current, frame averaging) demonstrated enhanced scan parameters at 90 kVp, 44 µA, 0.5 mm aluminum filter, 8 times frame averaging, and 4.9 µm voxel spacing. Post-processing with Richardson-Lucy deconvolution further deblurred the μCT images. Labeled microdamage in the baseline, enhanced and deblurred μCT images were segmented and spatially quantified vs. BSE-labeled microdamage using a probability-based correlation metric at six inflation radii. Enhanced μCT scan parameters improved damage visualization and increased spatial correlation probability with BSE images. Deblurring improved the sharpness of stain boundaries but did not significantly improve spatial correlation probabilities in comparison to the enhanced scans. This enhanced μCT protocol facilitates 3D visualization of microdamage, an indicator of bone quality important to bone damage mechanics.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.