Abstract
Background: Articular cartilage has a limited self-regenerative capacity, and tissue engineering is a promising solution to the problem of cartilage damage. Objective: The aim of this study was to evaluate the effect of hybrid microspheres (MS) composed of transforming growth factor (TGF)-β1-loaded gelatin MS and chitosan MS on enhancement of the differentiation of adipose-derived stem cells (ASCs) into chondrocytes in pellet culture. Methods: In vitro TGF-β1 release was evaluated by an enzyme-linked immunosorbent assay. The content of DNA and glycosaminoglycans (GAGs) was tested by biochemical methods. In addition, quantitative PCR was used to analyze the expression of collagen II and aggrecan. Results: Increased proliferation of ASCs was observed in the hybrid TGF-β1-loaded MS in comparison to the TGF-β1-loaded gelatin MS. The chondrogenic differentiation of ASCs in both constructs was evaluated, and GAG content and the gene expression of collagen II and aggrecan were significantly higher in the hybrid TGF-β1-loaded MS than in the TGF-β1-loaded gelatin MS. Conclusions: Enhanced differentiation of ASCs by hybrid TGF-β1-loaded MS may provide an easy and effective way to construct tissue-engineered cartilage.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
