Enhanced Chemiluminescence Determination of Hydrogen Peroxide in Milk Sample Using Metal–Organic Framework Fe–MIL–88NH2 as Peroxidase Mimetic

Abstract

The presence of hydrogen peroxide (H2O2) in milk is a major concern because it constitutes a public health hazard. Here, a sensitive flow injection chemiluminescence method was established for the detection of H2O2. As a peroxidase mimetic, metal–organic framework Fe–MIL–88NH2 was found to significantly enhance the chemiluminescence of luminol–H2O2 reaction. The enhancement mechanism could be attributed to peroxidase-like activity of Fe–MIL–88NH2, which effectively catalyzed the decomposition of H2O2 into hydroxyl radical. The experimental conditions for the chemiluminescence reaction were thoroughly investigated. The chemiluminescence intensity was proportional to the concentration of H2O2 in the range of 0.1–10.0 μmol/L. The detection limit was 0.025 μmol/L H2O2, and the relative standard deviation was 2.6 % for 11 replicated measurements of 1.0 μmol/L H2O2 solution. The practicability of this method was demonstrated by determining H2O2 in milk samples.