Abstract
The present study made an attempt to develop copper nanoparticles (Cu-NP) with antifungal property using green synthesis method. Copper oxide nanoparticles (CuO-NPs) botanically synthesized using Neem leaf extract (Azadirachta indica A. Juss) were characterized by using different techniques like; UV-visible spectrophotometry, FTIR, XRD, SEM and TEM. Materials were chosen the disease free and fresh Azadirachta indica A. Juss were collected and identified at Center of Biodiversity and Taxonomy. The plant samples were vigorously washed with distilled water then shade dried followed by sterilization with 0.1% mercuric chloride for 20s and again it was washed with distilled water. 15g powder form of plant material was added to 200ml double distilled, CO2 free and deionized water and kept in shaker at 80°C and 1500rpm for six hours. After agitation, the extract was separated by regular centrifugation at 10,000rpm followed by filtration by using whatmann filter paper. The final volume of 100ml of supernatant was collected as pure extract and stored in cool place for further use. The final results confirm a significant inhibition of CuO-NPs for the test fungi. Additionally, CuO-NPs demonstrated an enhanced effect when combined with Neem leaf extract. A total of 20-30% improvement in activity was noticed after combination, which correlates with commonly used synthetic fungicides. The toxicity results reveal that A. indica extract and their combined fractions with CuO-NP were less toxic to the test seeds of experimental plant while as bulk Cu followed by biosynthesized CuO-NPs influenced the germination rate as compared to control pots. The study drops a concern of research and offers a promising route of developing Copper based green fungicides that can help to combat with modern issues of synthetic fungicides. An average size of 80 ± 15nm monoclinic cupric oxide (CuO) and cubic cuprous oxides (Cu2O) nanocrystals that existed in mixed form were successfully developed.
Full Text
Topics from this Paper
Neem Leaf Extract
Synthesis Of Copper Oxide Nanoparticles
Whatmann Filter Paper
Ml Of Supernatant
Center Of Biodiversity
+ Show 5 more
Create a personalized feed of these topics
Get StartedTalk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Similar Papers
Materials Research Innovations
Aug 30, 2023
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Mar 1, 2014
Poultry science
Nov 1, 2023
African Scientist
Jun 30, 2023
Invertis Journal of Renewable Energy
Jan 1, 2020
Advances in Technology
Jun 8, 2021
Journal of Natural Remedies
Jun 1, 2006
JBIC Journal of Biological Inorganic Chemistry
Jun 22, 2019
Communications in Science and Technology
Dec 28, 2022
Journal of the American Oil Chemists' Society
Apr 1, 1990
Journal of Applied Research and Technology
Apr 27, 2023
Open Access Macedonian Journal of Medical Sciences
Jul 20, 2020
Colloids and Surfaces B: Biointerfaces
Jun 1, 2012
Brazilian Journal of Veterinary Research and Animal Science
Dec 23, 2020
Journal of Environmental Biology
Sep 2, 2023
Pharmaceutical Research
Pharmaceutical Research
Nov 17, 2023
Pharmaceutical Research
Nov 14, 2023
Pharmaceutical Research
Nov 7, 2023
Pharmaceutical Research
Nov 7, 2023
Pharmaceutical Research
Nov 3, 2023
Pharmaceutical Research
Nov 1, 2023
Pharmaceutical Research
Nov 1, 2023
Pharmaceutical Research
Nov 1, 2023
Pharmaceutical Research
Nov 1, 2023
Pharmaceutical Research
Nov 1, 2023