Abstract
The non-steroidal anti-inflammatory drug (NSAID) aspirin (acetylsalicylic acid) is an inhibitor of cyclooxygenase enzymes. Recent studies have shown that aspirin could be used as an anti-tumor drug. Triptolide, the major compound extracted from the Chinese herb Tripteryglum wilfordii Hook.f, has now been shown that it can inhibit tumor growth. The aim of this study was to analyze the anti-tumor efficiency of aspirin and triptolide in cervical cancer cells. Viability of cervical cancer cell lines was assessed by the MTT method at various concentrations of aspirin and triptolide. Siha and HeLa cell apoptotic analysis was performed by flow cytometry. Real time-PCR and Western Blotting were used to analyze the expression of Bcl-2/Bax, Cyclin D1 and p16. Viability in the combination group was significantly decreased as compared with either drug used alone. Expression change of Bcl-2/Bax, CyclinD1 and p16 appeared to play an important role in the synergistic killing effect on cervical cancer cell apoptosis. Aspirin and triptolide combination treatment may have synergistic anti-tumor effects on cervical cancer cells.
Highlights
Cervical cancer is the second most common cause of cancer and the fourth leading cause of cancer-related death in female all over the world
Combination of aspirin and triptolide suppress the proliferation of cervical cancer cell lines
Combined treatment with aspirin (5-10 μM) and VPA (50-100 nM) was assessed after 24h and 48 h, the combination of both agents exhibited a significantly synergistic anti-proliferation effect compared with drugs used separately (P < 0.01) (Figure 1C)
Summary
Cervical cancer is the second most common cause of cancer and the fourth leading cause of cancer-related death in female all over the world. Studies shown that aspirin could be used as an anti-tumor drug in several cancer cell lines (Xiaoxin et al, 2009; Ou et al, 2010; Park et al, 2010; Singh et al, 2010), but these anti-tumor efficiency is still low Another widely used drug, triptolide, the major composition extracted from the Chinese herb Tripteryglum wilfordii Hook.f, has been shown that it can inhibit tumor growth (Clawson et al, 2010; Zhao et al, 2010; Lu et al, 2011; Li et al, 2012; Zhu et al, 2012; Huang et al, 2013; Tan et al, 2013). Conclusion: Aspirin and triptolide combination treatment may have synergistic anti-tumor effects on cervical cancer cells
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