Enhanced anaerobic biodegradation of typical phenolic compounds in coal gasification wastewater (CGW) using biochar: Focusing on the hydrolysis-acidification process and microbial community succession

Abstract

The aim of this research is to investigate the effects of biochar (BC) on treatment performance (especially hydrolysis-acidification process) and microbial community shifts during anaerobic degradation of typical phenolic compounds in coal gasification wastewater. Compared to the control group, the removal of phenol, p-cresol and 3, 5-xylenol was gradually enhanced when increasing the BC addition within the test dosage (1–5 g/L). The biodegradation of phenol and p-cresol was significantly enhanced by BC addition while limited improvement for 3, 5-xylenol. The addition of BC significantly accelerated the hydrolysis-acidification process with the hydrolytic removal of phenol improved by 69.14%, the microbial activity was enhanced by 57.01%, and the key hydrolase bamA gene was enriched by 117.27%, respectively. Compared to 1–2 g/L dose, more protein-like and humic acid-like substances were secreted with 5 g/L BC, which probably contributed to higher extracellular electron transfer efficiency. In addition, phenol degrading bacteria (Syntrophorhabdus, Dysgonomonas, Holophaga, etc.) and electroactive microorganisms (Geobacter, Syntrophorhabdus, Methanospirillum, etc.) were enriched by BC addition. The functional genes related to carboxylation, benzoylation and ring cleavage processes of benzoyl-CoA pathway were potentially activated by BC.