Enhanced adventive embryogenesis resulting from plasmolysis of cultured wild carrot cells

Abstract

Adventive embryogenesis in vitro-grown somatic cells of Daucus carota L. was increased three-fold by a 45 min plasmolysis pre-treatment using 1M sucrose solutions. A high degree of synchronous development also resulted from this treatment. The enhancement of embryogenesis is interpreted as an increase in the regeneration of cells which have become physiologically somewhat isolated from the tissue of their origin by the plasmolysis-caused rupture of plasmodesmata. Possible causes of the increased synchrony are discussed.