Enhanced adaptation to salinity stress in lentil seedlings through the use of trehalose-functionalized silica nanoparticles (TSiNPs): Exploring silica-sugar absorption and oxidative balance

Abstract

Silica nanoparticles (SiNPs) confer better growth and development of plants under salinity stress. Moreover, the surface-functionalization of SiNPs with bioactive molecules is expected to enhance its efficacy. The present study thus aimed to modify the surface of SiNPs, by attaching a bioactive molecule (trehalose) to obtain TSiNPs. The successful surface functionalization was confirmed using FTIR, XRD, and EDS. The spherical shape and amorphous nature of the nanoparticles were confirmed using SEM. The TEM image analysis revealed that the size of SiNPs and TSiNPs ranged between 20-50 nm and 200–250 nm, respectively. A novel bioassay experiment designed to study the release of silica and trehalose from nanoparticles elucidated that the TSiNPs improved the release and uptake of silica. Also, trehalose uptake significantly improved after 72 h of application due to enhanced release of trehalose from TSiNPs. Further, this study also aimed to investigate the potential benefits of SiNPs and TSiNPs in promoting the growth and development of plants under salinity stress. In this context, the nanoparticles were applied to the saline-stressed (0, 200, 300 mM) lentil seedlings for the in-planta experiments. The results revealed that both SiNPs and TSiNPs improved the growth of seedlings (shoot, and root length), ionic balance (K+/Na+ ratio), and osmolyte status (sugars, proline, glycine betaine, trehalose). Additionally, increased antioxidant enzyme activities helped scavenge ROS (H2O2, O2.-) generated in NaCl-stressed seedlings, ultimately improving the membrane integrity (by reducing MDA and EL). However, the TSiNPs exhibited a much-enhanced activity in stress alleviation compared to the SiNPs.