Abstract
Abstract Aponin (an acronym for apparent Oceanic naturally occurring cytolyin) has been isolated from the green alga Nannochloris sp. following a previously described procedure, chloroform extraction of the cell‐free media. The crude material is cytolytically active toward the red tide organism Ptychodiscus brevis, an unarmored dinoflagellate responsible for red tides in west Florida coastal waters. The crude aponin was purified by means of reverse‐phase (C18) liquid column chromatography, and was tested by an improved bioassay. Under these conditions, a single‐step purification increases the specific activity (units/mg) to 2.5 × 105. The results are significant because some calculations of amount of aponin to treat a red tide have appeared that were based upon earlier work using crude aponin. Such calculations, obviously, give an incorrect, inaccurate, and biased view of the potential for management of a red tide.
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Schedule a callMore From: Journal of Environmental Science and Health . Part A: Environmental Science and Engineering
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