Abstract
We describe the use of spatially incoherent illumination to make quantitative phase imaging of a semi-transparent sample, even out of the paraxial approximation. The image volume electromagnetic field is collected by scanning the image planes with a quadriwave lateral shearing interferometer, while the sample is spatially incoherently illuminated. In comparison to coherent quantitative phase measurements, incoherent illumination enriches the 3D collected spatial frequencies leading to 3D resolution increase (up to a factor 2). The image contrast loss introduced by the incoherent illumination is simulated and used to compensate the measurements. This restores the quantitative value of phase and intensity. Experimental contrast loss compensation and 3D resolution increase is presented using polystyrene and TiO(2) micro-beads. Our approach will be useful to make diffraction tomography reconstruction with a simplified setup.
Highlights
Optical microscopy has been widely used for hundred of years for bio-medical imaging
We propose in this paper a simulation method to determine the modulation transfer function (MTF) of the couple microscope / quadriwave lateral shearing interferometer (QWLSI) for both intensity and optical path difference (OPD)
We demonstrated that single-shot quantitative phase imaging is possible even with incoherent illumination
Summary
Optical microscopy has been widely used for hundred of years for bio-medical imaging. Most of nonmodified biological samples are transparent at optical wavelengths, leading to low-contrast images when using a conventional intensity-sensitive sensor on a microscope. In this paper we propose to study quantitative phase imaging under any illumination spatial coherence using a quadriwave lateral shearing interferometer (QWLSI) [15]. Theoretical MTF is well known in conventional intensity imaging setup [18] because it allows quantitative interpretation of the measurements. It can be generalized in 3D and for phase-sensitive imaging setup [19]. We validate our approach with point-like objects and show that this technique drastically increases phase and intensity image 3D-resolution
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