Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis.

Yoshimasa Imoto,Masanori Kidoguchi,Kanako Yoshida,Naoto Adachi,Masafumi Sakashita,Masafumi Kanno,Tetsuji Takabayashi,Yukinori Kato,Yumi Ito,Norihiko Narita,Kazuhiro Ogi,Yukihiro Kimura,Keisuke Koyama,Shigeharu Fujieda,Masayuki Okamoto

doi:10.3390/biom10111568

Yoshimasa Imoto, Masanori Kidoguchi + Show 13 more

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https://doi.org/10.3390/biom10111568

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Journal: Biomolecules	Publication Date: Nov 18, 2020
Citations: 20	License type: CC BY 4.0

Affiliation: University of Fukui

Abstract

Background: The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have significant roles in allergic disease; however, the significance of 15-LOX-1 in CRS is not well understood. The objective of this study was to demonstrate the expression of 15-LOX-1 in CRS. Methods: The mRNA expression levels of 15-LOX-1 and periostin in nasal tissues were measured by quantitative real-time polymerase chain reaction. We also performed an immunofluorescence study of nasal tissues. Cells of the Eol-1 eosinophilic leukemic cell line were stimulated with interleukin-33 to test the induction of 15-LOX-1. Results: The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Moreover, interleukin-33 enhanced 15-LOX-1 expression in Eol-1 cells. Conclusions: 15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS.

Highlights

Chronic rhinosinusitis (CRS) is a disease that is defined by inflammation of the nose and paranasal sinuses that lasts for at least 12 weeks and causes nasal obstruction, facial pain, olfactory dysfunction, and mucopurulent drainage [1,2,3]
As the immunofluorescence assay revealed that eosinophils expressed 15-LOX-1, we investigated the correlation between the number of eosinophils and the mRNA expression level of 15-LOX-1 in nasal polyps (NPs)
Since the immunohistochemistry analysis showed that epithelial cells expressed 15-LOX-1 in sinonasal tissues, especially in the NPs from eosinophilic CRS (ECRS) patients, we investigated the induction of 15-LOX-1

Summary

Introduction

Chronic rhinosinusitis (CRS) is a disease that is defined by inflammation of the nose and paranasal sinuses that lasts for at least 12 weeks and causes nasal obstruction, facial pain, olfactory dysfunction, and mucopurulent drainage [1,2,3]. In Western countries, CRSwNP tissues often display intense eosinophilic infiltration and a type biased cytokine profile [8,10]; in contrast, in Asia, they are more often characterized by neutrophil-dominant inflammation [11,12]. The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Results: The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Conclusions: 15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS

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