Abstract

Recently, chromogenic media were used for rapid detection of pathogenic agents isolated from different materials (food, water and stool). Results of the present study indicated that specific and rapid identification of Enterobactiriaceae isolates from 46 food samples (egg, dairy products, meat, salads and cooked rice) as compared to conventional methods of biochemical test and plating selective media consume times, are expensive , autoclaving, and less selective. Both media ECC Agar (HIMEDIA) and Salmonella differential agar (Raj Hans Medium, HIMEDIA) are powerful for screening isolates from food samples: Esherichia coli, Salmonella spp., Klebsiella pneumoniea. Key words: Chromogenic media, Enterobacteriaceae, detection, food.

Highlights

  • Enterobacteriaceae, Esherichia coli coliform are the most infectious bacteria of foodstuff products

  • Results of the present study indicated that specific and rapid identification of Enterobactiriaceae isolates from 46 food samples as compared to conventional methods of biochemical test and plating selective media consume times, are expensive, autoclaving, and less selective

  • The goal of this study was to evaluate the efficiency of two chromogenic media in detecting and isolating Enterobacteriaceae from food samples

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Summary

Introduction

Enterobacteriaceae, Esherichia coli coliform are the most infectious bacteria of foodstuff products. Traditional methods for bacterial detection like biochemical test have been used for long. Previous study on Coliforms and specially E. coli made it possible to identify them as microbial contaminants marker in food and water. Their presence in drinking water and food indicates that these materials are contaminated with other enteric pathogens. Their isolation and enumeration have great importance in the determination of food hygiene (Muller et al, 2001).

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