Abstract
The objective of this study was to use enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindrome PCR (REP-PCR) for the analysis of genetic diversity among Escherichia coli strains isolated from commercial swine farms in Sichuan province of China. Thirty four strains of E. coli were selected by selective medium and conventional biochemical test from fresh stool samples of swines in five farms in Sichuan province. The isolates were identified by 160 kinds of E. coli O serums. The results show that 30 strains were determined among 34 E. coli isolates, 12 kinds of O serogroups were obtained on the basis of the agglutination test. The predominant types are O23, O113 and O120, representing 35.4%. Furthermore, the genotypes and phylogenetic relationship of all isolates were analysed by Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindrome PCR (REP-PCR), 34 E. coliisolates were clustered to 19 ERIC-PCR genotypes and 13 REP-PCR genotypes. The isolates from the same farm or sharing the same serotyping showed different genotype. And the isolates which could not be serotyped were genotyped by ERIC-PCR and REP-PCR. The analysis of genetic type and original source revealed that isolates from different farms had different genetic types. The subtypes of E. coli are also different within a single farm. Genetic variability with E. coli strains isolated from swine farms in China has been demonstrated. The presence of ERIC-PCR and REP-PCR sequences in the genome of E. coli was confirmed. ERIC-PCR and REP-PCR techniques are more rapid methods for molecular typing of E. coli strain. They are also useful methods for diversity survey of E. coli and the two methods analyzes genetic diversity of E. coli isolated in Sichuan of China. Key words: Escherichia coli, serotype, enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), repetitive extragenic palindrome PCR (REP-PCR).
Highlights
Escherichia coli is one of the strain widely distributed in nature and intestinal tract of animal
The objective of this study was to use enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindrome PCR (REP-PCR) for the analysis of genetic diversity among Escherichia coli strains isolated from commercial swine farms in Sichuan province of China
The genotypes and phylogenetic relationship of all isolates were analysed by Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and repetitive extragenic palindrome PCR (REP-PCR), 34 E. coli isolates were clustered to 19 ERIC-PCR genotypes and 13 REP-PCR genotypes
Summary
Escherichia coli is one of the strain widely distributed in nature and intestinal tract of animal. Genetic diversity exists widely among strains, so that using traditional bacterial taxonomy proves problematic when differentiating between strains that share a close genetic relationship. The traditional method for subspecies typing among the E. coli has been serotyping. The serotype is based on the antigenic properties of the O-antigen (surface polysaccharide) and H antigen(s) (flagellar). Typing the O-antigen itself denotes the serogroup, and
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