Abstract
Phyllanthus odontadenius Müll. Arg is one of the most important medicinal plants in the genus Phyllanthus (Phyllanthaceae). It is used in different regions in the world for the treatment of various diseases for example malaria caused by Plasmodium falciparum causing millions deaths in tropical and subtropical regions. In this work, we experimented P. odontadenius seeds germination and seedlings infected by Agrobacterium rhizogenes A4RS/pHKN9. We obtained 36% of seeds that germinated for 84.6% of seedlings transformed which showed positive gfp activities. The number of hairy roots formed in infectious sites for seedlings infected with A. rhizogenes A4RS/pHKN29 is valued at 6.3±2.71 against 0 for control seedlings. Diagrams showed that mean length for the new-formed roots were 1.92±0.55 cm and 1.59±0.49 cm for hairy root. Key words: Phyllanthus odontadenius, hairy roots, Agrobacterium rhizogenes, malaria, secondary metabolites.
Highlights
In many tropical and subtropical regions, malaria remains one of the main global health problems of our time, causing more than 1 million deaths per year, with about90% of deaths and 60% cases occurring in South Africa in the Sahara
Others protocols used have not produce germination or seedlings because sulphuric acid showed a fatal effect on P. odontadenius seeds germination and on the other hand, gibberellic acid (GA3) has positive or stimulate effect in raising of
The sulphuric acid toxicity when it is used in P. odontadenius seeds scarification which did not germinate after their dipping in gibberellic acid solution
Summary
In many tropical and subtropical regions, malaria remains one of the main global health problems of our time, causing more than 1 million deaths per year, with about. 90% of deaths and 60% cases occurring in South Africa in the Sahara. It is caused by the protozoan parasite. A. rhizogenes mediated transformation has been used to produce transgenic hairy root culture and plantlets have been regenerated (Anand, 2010). Several studies on A. rhizogenes (Tepfer, 1983, 1984, 1990; Chabaud et al, 2006) have shown that many dicotyledonous plants are susceptible to infection by these bacteria and can be transformed and produce hairy roots, some of these species have no ability to regenerate plantlets through their hairy roots
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