Abstract
An F2 population derived from the hybrid of Lycopersicon esculentum Mill.XF98-7×Lycopersicon pimpinellifolium LA2184 was used for genome-wide linkage analysis for yield traits in tomato. The genetic map, spanning the tomato genome of 808.4 cM long was constructed with 112 SSR markers distributing on 16 linkage groups. Main and epistatic effect QTLs controlling first flower node, number of flowers per truss, fruit set percentage and fruit weight were located using Bayesian model selection method. A total of 20 significant main effect QTLs and 16 pairs of epistatic QTLs were identified on 16 linkage groups. The proportions of phenotypic variation explained by the detected QTLs ranged from 1.9 to 25.9% and from 0.00 to 17.4% for main-effect and epistatic QTLs, respectively. Most QTL effects were predictable from the parental phenotypes. Additionally, one QTL was found to be pleiotropic, governing simultaneously first flower node and number of flowers per truss. Key words: Tomato, SSR marker, yield traits, QTL, Bayesian model selection.
Highlights
Construction of a high density genetic map is the foundation of gene localization, gene cloning and structural and functional genome research
The earliest high density genetic map covering the tomato genome of 1276 cM long has been established by Tanksley et al (1992) with 1030 restriction fragment length poly-morphism (RFLP) markers in an F2 population developed from a cross between an elite line of tomato (L. esculentum)
Fulton et al (1997) have detected 8 quantitative trait loci (QTLs) for fruit weight, whose reliability has been verified in the BC2, BC3 and BC4 progenies in tomato
Summary
Construction of a high density genetic map is the foundation of gene localization, gene cloning and structural and functional genome research. The earliest high density genetic map covering the tomato genome of 1276 cM long has been established by Tanksley et al (1992) with 1030 restriction fragment length poly-morphism (RFLP) markers in an F2 population developed from a cross between an elite line of tomato (L. esculentum). Foolad and Chen (1998) have published a linkage map of approximate 600 cM length, which has been constructed with 53 Random amplification of polymorphic deoxyribonucleic (RAPD) markers in F2 population derived from UCT5 (L. esculentum) and LA716 (L. pennellii). The first flower node, the number of flowers per truss, fruit set percentage and fruit weight are the important traits for the production of tomato These traits are typically controlled by multiple genes, often collectively referred to as
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