Abstract
Two Azotobacter strains were isolated from alkaline and acid soils. The production of alginate and exopolymer from these two strains showed that, strain AC2 produced high polymer in 2% beet molasses or 1% sucrose broth and addition of nitrogen sources (yeast extract) reduced production of this polymer. The optimum condition for production of maximum polymer production (7.5 mg/ml) was at 200 rpm shaking, pH 7 without addition of nitrogen sources. The production of polymer was reduced at pH 4. The polymer adsorbed Cu, Zn, and Fe, at 15.5, 20 and 25 mg, respectively. Key words: Azotobacter, exopolymer, of extracellular polysaccharide, biosorption of metal.
Highlights
Production of extracellular polysaccharide had been studied in Pseudomonas aeruginosa (Castaneda et al, 2000) Erwinia, (Deretie et al, 1987) Ralstonia (Dolph et al, 1988; Kao et al, 1992), and Azotobacter vinelandii (Saile et al, 1997)
Extracellular polysaccharide is required for wild-type virulence of Pseudomonas solanacearum and other microorganisms (Willis et al, 2001)
Extracellular polysaccharide from Azotobacter is used to biosorbe metals
Summary
Production of extra-cellular polymer in Azotobacter and biosorption of metal by exopolymer. Giti Emtiazia*, Zahra Ethemadifara and Mohammad H. Habibib aBiology Department, Isfahan University, Isfahan, Iran 81745-117. Two Azotobacter strains were isolated from alkaline and acid soils. The production of alginate and exopolymer from these two strains showed that, strain AC2 produced high polymer in 2% beet molasses or 1% sucrose broth and addition of nitrogen sources (yeast extract) reduced production of this polymer. The optimum condition for production of maximum polymer production (7.5 mg/ml) was at 200 rpm shaking, pH 7 without addition of nitrogen sources. The production of polymer was reduced at pH 4. The polymer adsorbed Cu, Zn, and Fe, at 15.5, 20 and 25 mg, respectively
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