English

Amina N Djemel,Nadia Ykhlef,Nadir Belbekri,Mahbouba Benmati,Abdelhamid Djekoun

doi:10.5897/ajb12.2817

Abstract

Frankia strains can induce N2-fixing root nodules on certain non-leguminous plants. It is known that exogenous application of 2,4-dichlorophenoxyacetate (2,4-D) affects root morphology. In this work, wheat roots were treated with 2,4-D and inoculated with the actinomycete Frankia. Wheat plants grew in a growth chamber with hydroponic medium. Binocular observation revealed that para-nodules were formed when wheat roots were inoculated with Frankia and the root length was enhanced. When the inoculation with Frankia was combined to 2,4-D treatment, the para-nodules formed were bigger and more numerous, while the root length was shortened. Keywords: Frankia , wheat, roots, para-nodules, 2,4-dichlorophenoxyacetate African Journal of Biotechnology Vol. 12(35), pp. 5427-5430

Highlights

Actinomycetes are found in soil, rhizosphere, ponds and lake sediments
Binocular observation revealed that para-nodules were formed when wheat roots were inoculated with Frankia and the root length was enhanced
When combined with 2,4-D treatment, the root length was considerably shortened (Figure 1), and the lateral ones were hardly absent. These results are in accordance with those of El-Shahed and Abdel-Wahab (2006), who established that different 2,4-D concentrations in combination with Nostoc rivulare decrease root length in wheat, maize and rice, while the simple application of N. rivulare significantly enhances shoot and root weight as well as plant height as we observed in this work with Frankia

Summary

Introduction

Actinomycetes are found in soil, rhizosphere, ponds and lake sediments. They are a large group of bacteria that can produce secondary metabolites which have important applications in pharmacy, medicine and agriculture (Suttiviriya et al, 2008). Wheat roots were treated with 2,4-D and inoculated with the actinomycete Frankia. Binocular observation revealed that para-nodules were formed when wheat roots were inoculated with Frankia and the root length was enhanced.

Results

Conclusion