Abstract
Three R2R3-MYB genes, designated CaMYB1, CaMYB2 and CaMYB3, were isolated from hot pepper (Capsicum annuum. L). CaMYB1, CaMYB2 and CaMYB3encode polypetides consisting of 340, 262 and 345 amino acids respectively, containing R2R3 domain and the signature motif specific for the interaction between MYB and bHLH proteins in the R3 domain. Phylogenetic analysis based on the deduced amino acid sequences of these three R2R3 MYB transcription factor members revealed that CaMYB1 and CaMYB2 clustered together with the anthocyanin-related subgroup of R2R3 MYB proteins from other plants, while CaMYB3 did not. CaMYBs transcripts accumulation was detected in all stages of fruit development and in flower and leaves. Three CaMYBs transcription factors showed differential expression during fruit ripening. Anthocyanin biosynthetic gene expression patterns were quite different in young leaves, flower, and the four stages of fruit development. CaMYB1 and CaMYB2 may regulate anthocyanin biosynthesis in hot pepper. Key words: Anthocyanin, Capsicum annuum, gene expression, R2R3 MYB transcription factor.
Highlights
The MYB transcription factors family is one of the most abundant classes of transcription factors in plants (Rosinski and Atchley, 1998)
Phylogenetic analysis based on the deduced amino acid sequences of these three R2R3 MYB transcription factor members revealed that CaMYB1 and CaMYB2 clustered together with the anthocyanin-related subgroup of R2R3 MYB proteins from other plants, while CaMYB3 did not
We reported on the cloning and characterization of three genes encoding R2R3 MYB protein
Summary
The MYB transcription factors family is one of the most abundant classes of transcription factors in plants (Rosinski and Atchley, 1998). MYB transcription factors are classified into three sub-families; MYB1R, MYBR2R3 and MYB3R factors; depending on the number of imperfect repeats (R1, R2 and R3) in the DNA-binding domain, each consisting of about 50 amino acids and including a helix-turn-helix structure Among these MYB transcription factors, R2R3-MYBs constitute the largest transcription factors gene family in plants, with 126 R2R3 MYB genes identified in Arabidopsis (Stracke et al, 2001).
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