Abstract
This study evaluated the potential use of proteases produced by Erwinia chrysanthemi for the extraction of pure chitin from crustacean wastes using standard methods. The crude bacterial protease had activity of 22.4 U/ml. Protein removal by the commercial protease were similar in both the mineralized and demineralized wastes and amounted to about 75 and 80%, respectively, in 16 h. A similar trend was observed with higher protein removal efficiency (95%) for the crude protease from both the mineralized and demineralized wastes. Also, treatment of the powder with 5% NaOH resulted in the removal of protein in the demineralized and mineralized wastes decreased by about 96 and 87.6%, respectively, in same period from an initial concentration of 0.3 mg/ml. It was concluded that proteases produced by E. chrysanthemi could be used to transform waste from sea-food processing industries into products of commercial value. Key words: Erwinia chrysanthemi, extra cellular, proteases, chitin, value-added products.
Highlights
Crustacean shells are major wastes of seafood processing industries and they tend to cause unpleasant odors or environmental pollution if not adequately processed
This study evaluated the potential use of proteases produced by Erwinia chrysanthemi for the extraction of pure chitin from crustacean wastes using standard methods
Since crustacean wastes are rich in such valuable substances such as protein, astacene, chitin and calcium, they could be used to produce high valueadded materials if recycled
Summary
Evaluation of proteases produced by Erwinia chrysanthemi for the deproteinization of crustacean waste in a chitin production process. This study evaluated the potential use of proteases produced by Erwinia chrysanthemi for the extraction of pure chitin from crustacean wastes using standard methods. Protein removal by the commercial protease were similar in both the mineralized and demineralized wastes and amounted to about 75 and 80%, respectively, in 16 h. A similar trend was observed with higher protein removal efficiency (95%) for the crude protease from both the mineralized and demineralized wastes. Treatment of the powder with 5% NaOH resulted in the removal of protein in the demineralized and mineralized wastes decreased by about 96 and 87.6%, respectively, in same period from an initial concentration of 0.3 mg/ml. It was concluded that proteases produced by E. chrysanthemi could be used to transform waste from sea-food processing industries into products of commercial value
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
