Abstract
Developing a protocol for somatic embryogenesis (SE) for adult mother plants of Jatropha curcas, aids to overcome problems such as harvesting time and an uneven yield, providing the opportunity to propagate proven elite genotypes. Until now, several authors have achieved SE in J. curcas using different explants, however, in none of these protocols adult plants have been used as mother plant material. Furthermore, the challenge of overcoming the morphogenesis limitations successfully and further regeneration of adult plants of J. curcas has been great. The main objective of this study was to develop a somatic embryogenesis protocol for adult plants of the oil producing J. curcas. transverse thin cell layers (tTCL) of young petioles were used as explants. Half strength Y3 and MS media were used containing three different concentrations of 2,4-dichloropherioxyacetic acid and 6-benzil-aminopurine in a factorial trial. Nine accessions of J. curcas of various ages (3-12 years.) were tested using this protocol. All accessions responded positively to callus induction and to the induction of somatic embryos, demonstrating the protocol to be genotype-independent. This study enables micropropagation of adult of proven elite plants of J. curcas via somatic embryogenesis. Key words: Jatropha curcas, transverse thin cell layers (tTCL), somatic embryos, half strength media, regeneration.
Highlights
In a world of an increasing demand for energy, fossil fuels are becoming more scarce and less favourable due to their CO2 emissions and impact on global warming (Rehman et al, 2019)
The main objective of this study was to develop a somatic embryogenesis protocol for adult plants of the oil producing J. curcas. transverse thin cell layers of young petioles were used as explants
This study enables micropropagation of adult of proven elite plants of J. curcas via somatic embryogenesis
Summary
In a world of an increasing demand for energy, fossil fuels are becoming more scarce and less favourable due to their CO2 emissions and impact on global warming (Rehman et al, 2019). The focus on renewable energies has identified Jatropha curcas L. as a promising plant to contribute to a more sustainable alternative. This is due to the yield and quality of the oil extracted from the seeds, having a relatively high oil content, ranging from ~30% to ~35% (Barros et al, 2015; de Oliveira et al, 2009).
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