Abstract
To study the effects of Mesima Reishi UE-1 on the metastatic phenotype of the human ovarian cancer cell line HO8910. HO8910 cells were pretreated with different concentration of Mesima Reishi UE-1 in vitro. Using cell proliferation assay, spreading and adhesion assay, cell migration and invasion assay, zymography, immunocytochemical staining and RT-PCR, we examined the effects of Mesima Reishi UE-1 on cell proliferation, motility, adhesion and expression of MMP-2, 9 invitro. Mesima Reishi UE-1 directly inhibited HO8910 cell proliferation in vitro. And pretreated with different concentrations of Mesima Reishi UE-1, HO8910 cells motility, adhesion and invasion were inhibited significantly. The activity of MMP-9 was inhibited in a dose-dependent manner. The protein and mRNA expression of MMP-2, 9 were also inhibited. Mesima Reishi UE-1 suppresses invasion of human ovarian cancer cells in vitro. Key words: Mesima Reishi UE-1, polysaccharides, human ovarian cancer, tumor invasion, matrix metalloproteinase.
Highlights
Human ovarian cancer, which occurs mainly in postmenopausal women, is the leading cause of death from gynecological malignancy.Since ovarian cancer often remains clinically silent, the majority of patients with ovarian carcinoma have advanced intraperitoneal metastatic disease at diagnosis, resulting in a poor prognosis
Using cell proliferation assay, spreading and adhesion assay, cell migration and invasion assay, zymography, immunocytochemical staining and RT-PCR, we examined the effects of Mesima Reishi UE1 on cell proliferation, motility, adhesion and expression of Matrix metalloproteinase (MMP)-2, 9 in vitro
HO8910 cells were cultured in 10% fetal bovine serum (FBS) high glucoseDulbecco modified eagle medium (H-DMEM) containing Mesima Reishi UE-1 0, 100 and 200 mg/l for 48 h
Summary
Human ovarian cancer, which occurs mainly in postmenopausal women, is the leading cause of death from gynecological malignancy (http://www.ovariancancer.org/about-ovarian-cancer/statistics/accessed 10/19/09).Since ovarian cancer often remains clinically silent, the majority of patients with ovarian carcinoma have advanced intraperitoneal metastatic disease at diagnosis, resulting in a poor prognosis. Human ovarian cancer, which occurs mainly in postmenopausal women, is the leading cause of death from gynecological malignancy About 80% of ovarian cancer cases are diagnosed at an advanced stage after metastasis has occurred Developing and exploiting Chinese herbal medicines to treat cancer has become a hot spot in new anti-cancer. He et al 9889 prolong patients’ survival time (Xu et al, 2011; Hu et al, (2002). The structure of a portion of water-soluble mycelial polysaccharide from P. linteus was a core (1-3)-linked glucan heavily substituted via (1-6) links with (1-3) linked mannose chains (Baker et al, 2008). During the past few decades, it has been found that polysaccharides isolated from P. linteus have antitumor and immunemodulating activities (Moradali et al, 2007; Zhang et al, 2007; Li et al, 2004; Kim et al, 2006; D Sliva et al, 2008)
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