Abstract
Melia dubia Cav. (Meliaceae) is a multipurpose tree of tropical and subtropical regions mainly cultivated for its medicinal and industrial importance. Due to its versatile properties, it has been depleted in its natural environment. Moreover due to sluggish and poor seed germination, there is a threat of its gene pool exclusion from the natural habitat. The alternative method for conservation and efficient mass propagation is thus need of the hour. As per the extensive literature survey there is no report on efficient protocol for mass propagation of M. dubia through callus organogenesis. Therefore, the present work was aimed to develop in vitro organogenesis protocol for rapid and large scale production of planting material. From our results, maximum callus percentage, callus weight and fragile callus was observed on 1.0 mg/l benzylaminopurine (BAP) in combination with 0.5 mg/l naphthalene acetic acid (NAA). The callus differentiation was achieved at different concentrations of BAP and indole acetic acid (IAA). Multiple Shoot number per callus propagule 5.30 was observed on 0.5 mg/l BAP and 1 mg/l IAA concentration. The maximum rooting percentage (78.5%), root number per explant (4.33) and root length per explant (4.41 cm) was observed at 0.5 mg/l indol butyric acid (IBA) after 30 days of inoculation. Further the total flavonoid content, phenolic content and antioxidant properties of leaves of in-vitro regenerated plants where studied. Total flavonoids and phenolic content in leaves of in vitro Melia dubia was 0.56 ± 0.8 mg quercitin equivalent (QE) and 2.97 ± 0.17 mg gallic acid equivalent (GAE) respectively. The antioxidant property was further assed through measurement of DPPH radical scavenging activity. The in-vitro regeneration protocol can be exploited for commercial cultivation and fulfilling the growing demand for fresh explant material through mass propagation of M. dubia an economically important plant species. Key words: Melia dubia, antioxidant, indole-3-butyric acid, flavonoids and phenolics.
Highlights
Melia dubia, a dicotyledonous multipurpose tree belonging to family Meliaceae, has huge commercial and industrial potential
As per the extensive literature survey there is no report on efficient protocol for mass propagation of M. dubia through callus organogenesis
The IC50 values were calculated as the concentration of extracts causing 50% inhibition of DPPH radical; a lower IC50 value corresponds to a higher antioxidant activity of sample
Summary
A dicotyledonous multipurpose tree belonging to family Meliaceae, has huge commercial and industrial potential. The purpose of micropropagation is to develop physiologically stable plantlets which can be acclimatized in a reduced time period and at a lower cost In this backdrop, we have established a well developed in vitro regeneration system for M. dubia for rapid and large scale production of planting material. The ethanolic extract of field grown plants has promising antioxidant activity with IC50 (16.89 μg/ml) value (Valentina et al, 2013) In this direction, the present study was envisaged to evaluate the antioxidant activities of leaf tissue from in vitro regenerated plants of M. dubia. The present study was envisaged to evaluate the antioxidant activities of leaf tissue from in vitro regenerated plants of M. dubia This activity can be ascribed to the phenolic and flavonoid compounds present in the species. The tissue culture raised plants were further evaluated for their total phenolic and flavonoid content viz-a-viz its antioxidant property
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