English

Singh Paramdeep,Meeta Jindal Madhu,Rani Ritu,Kumar Bipen

doi:10.5897/ajmr2014.6996

Abstract

Different inoculation techniques viz. inoculation of sets by dipping in spore suspension, bud inoculation with hypodermic syringe, bud wrapping by cotton swab dipped in smut suspension and inoculation of underground bud at the time of tillering (end May) were tested to screen against sugarcane smut in the field as well as in the laboratory conditions. In the field, out of the tested inoculation techniques, the maximum disease incidence (60.63%) was observed when buds were inoculated with hypodermic syringe and minimum (8.55%) in inoculation of underground buds at the time of tillering stage. The result showed that inoculation through mechanical injury significantly increase disease incidence but at the same time also affect the bud germination (15.56%). Significant difference in smut incidence was also observed when the inoculation was carried by teliospores and sporidia separately. Key words: Ustilago scitaminea, inoculation technique, screening, resistance, sugarcane.

Highlights

Different in noculation techniques t viz. inocula ation of setts by dippiing in sporre suspensiion, bud inoculation with hypod dermic syring ge, bud wra apping by co otton swab d dipped in sm mut suspens sion and inoculation of undergrround bud at a the time of tillering were tested d to screen n against sugarcane smut in the e field as well w as in the e laboratory y conditions s
Out of th he tested inoculation techniques s, the maxim mum diseas se incidence e (60.63%) w was observe ed when bu uds were inoculated with hypode ermic syring ge and minim mum (8.55%)) in inoculattion of unde erground bud ds at the time of tillering stage
Fungal disease d like red r rot, smut and wilt are of majo or importance e and are mainly responsible for reducing r the yield y and deterioration of the t juicce quality

Summary

Introduction

Different in noculation techniques t viz. inocula ation of setts by dippiing in sporre suspensiion, bud inoculation with hypod dermic syring ge, bud wra apping by co otton swab d dipped in sm mut suspens sion and inoculation of undergrround bud at a the time of tillering (end May) were tested d to screen n against sugarcane smut in the e field as well w as in the e laboratory y conditions s. Significant difference in i smut incid dence was a observe ed when the e inoculation n was carried d by teliospo ores and sporidia separately. Field experiment on testing of four inoculation techniques with two types of spores (teliospores and sporidia) of U. scitaminea was conducted at University Seed Farm, Ladhowal, Punjab Agricultural One hundred and thirty five single budded sets of mid-season sugarcane smut susceptible variety CoJ 88 were used for each method of inoculation.

Results

Conclusion