Abstract
Salmonella is a globally widespread food-borne pathogen having major impact on public health. Salmonellosis is an infection with bacteria called Salmonella. Most persons infected with Salmonella develop diarrhea, fever, and abdominal cramps 12 to 72 h after infection. This study was designed to isolate and identify Salmonella spp. from cloacal swabs of apparently healthy broiler chickens in Bangladesh. Salmonella was characterized culturally, biochemically and also via PCR method. Among 50 isolates, 16 were found to be positive for Salmonella. PCR using 16S rRNA gene primers produced a 496 bp band indicating positive result for Salmonella spp. Antibiotic sensitivity test using six commonly used antibiotics in Bangladesh named colistin sulphate, erythromycin, cloxacillin, ciprofloxacin, neomycin and amoxicillin demonstrated that 5 (31.25%) strains were resistant towards ciprofloxacin. Fourteen (87.5%) isolates were resistant to amoxicillin, while fourteen (87.5%) were found intermediate towards neomycin. The study revealed that, healthy broiler chicken sold at local markets of Bangladesh transmit drug resistant Salmonella to the environment, therefore, use of antibiotics can be monitored in food producing animals since drug resistance could be a major public health problem in developing countries like Bangladesh. Key words: Antibiogram, Salmonellosis, PCR, broiler chicken, drug resistance.
Highlights
Salmonella is considered the most important food-borne bacterial pathogen in the world (Foley et al, 2011), this is a Gram negative, short rod-shaped, non-spore forming, non-capsulated, facultative anaerobic bacterium (OIE, 2006)
The study revealed that, healthy broiler chicken sold at local markets of Bangladesh transmit drug resistant Salmonella to the environment, use of antibiotics can be monitored in food producing animals since drug resistance could be a major public health problem in developing countries like Bangladesh
Among 16 isolates 10 isolates were found to be non motile characterized by forming the stab line without producing turbidity in the Motility Indole Urea (MIU) medium and another 6 isolates were found motile characterized by changing of colour of MIU medium
Summary
Salmonella is considered the most important food-borne bacterial pathogen in the world (Foley et al, 2011), this is a Gram negative, short rod-shaped, non-spore forming, non-capsulated, facultative anaerobic bacterium (OIE, 2006). The reasonably surveillance for Salmonella is to reduce human Salmonellosis of poultry origin across the world (Keery, 2010). Salmonella is grouped into three main classes based on their association with human and animal hosts.
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