Abstract
The water-soluble polysaccharide (GLP) was obtained from the spores of Ganoderma lucidum by hot water extraction and gel chromatography. The molecular weight was estimated to be 143 KD determined by using HPGPC. Structure features of GLP were investigated by a combination of chemical and instrumental analysis. The results indicated that GLP consisted of a backbone composed of (1→6)- linked-α-d-glucopyranosyl, (1→3)-linked-β-d-glucopyranosyl and (1→3,6)-linked-β-d-glucopyranosyl residues in the ratio of 1:2:1, and terminated with one single terminal (1→)-β-d-glucopyranosyl at the O- 6 position of (1→3,6)-linked-β-d-glucopyranosyl, along the main chain. Preliminary tests in vitro showed that GLP has stimulating effects on murine lymphocyte proliferation induced by concanavalin A (ConA) and lipopolysaccharide (LPS) in a dose-dependent manner. It is a possible potential immunopotentiating agent for use in functional foods or medicine against both pathogens and cancer. Key words: Ganoderma lucidum, polysaccharide, structure, lymphocyte proliferation.
Highlights
Many polysaccharides have been isolated from some natural materials including mushrooms, fungi, yeasts, algae, lichens and plants, and their biological activities have attracted more attention in the biochemical and medical areas due to their immunomodulatory and anti-cancer electrochemotherapy (ECT) (Ooi and Liu, 2000; Wasser, 2002)
The results indicated that GLP consisted of a backbone composed of (1 6)linked- -d-glucopyranosyl, (1 3)-linked- -d-glucopyranosyl and (1 3,6)-linked- -d-glucopyranosyl residues in the ratio of 1:2:1, and terminated with one single terminal (1 )- -d-glucopyranosyl at the O6 position of (1 3,6)-linked- -d-glucopyranosyl, along the main chain
Preliminary tests in vitro showed that GLP has stimulating effects on murine lymphocyte proliferation induced by concanavalin A (ConA) and lipopolysaccharide (LPS) in a dose-dependent manner
Summary
Many polysaccharides have been isolated from some natural materials including mushrooms, fungi, yeasts, algae, lichens and plants, and their biological activities have attracted more attention in the biochemical and medical areas due to their immunomodulatory and anti-cancer electrochemotherapy (ECT) (Ooi and Liu, 2000; Wasser, 2002). The water-soluble polysaccharide (GLP) was obtained from the spores of Ganoderma lucidum by hot water extraction and gel chromatography. Preliminary tests in vitro showed that GLP has stimulating effects on murine lymphocyte proliferation induced by concanavalin A (ConA) and lipopolysaccharide (LPS) in a dose-dependent manner.
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