Abstract
Seed germination, seedling growth and in vitro regeneration studies were conducted on H. floribunda(Apocynaceae), a potential reforestation candidate. The seeds germinated readily with 96.0 ± 2.5% germination in 10 days. The seedlings exhibited a slow growth rate, attaining a shoot height of 35.5 ± 4.46 cm and a leaf area of 23.86 ± 3.89 cm2 in 12 weeks. Murashige and Skoog (MS) medium supplemented with various concentrations of 2, 4-dichlorophenoxyacetic acid, (2, 4-D) alone or combined with Benzyladenine induced callus formation in leaf, internode and node explants. Massive callus production was generally observed with explants cultured on MS media supplemented with 4.5 µM 2, 4-D alone or combined with 2.2 µM BA. The highest frequency of shoot regeneration was induced by a combination of 4.5 µM 2, 4-D and 2.2 µM BA; no complete plantlets were however produced. Key words: Callus, germination, H. floribunda, plant regeneration, seedling growth.
Highlights
This paper reports the seed germination and seedling growth of H. floribunda and the preliminary experiments carried out on the development of protocol for callus induction and multiple shoot production from its various explants
It was observed that 2.5% of the seeds germinated by the second day of incubation and the germination progressed with time reaching 77.5% by day 8 and 96% by day 10
The high percentage germination observed for H. floribunda seeds shows that the seeds are not dormant
Summary
It is a small to medium size tree (10 to 15 m) depending on the ecological zones (Tamboura et al, 2005). It is a highly valued multipurpose tree. The white wood of H. floribunda is used for carvings and the floss obtained from the seeds serve for stuffing pillows. One of several reasons is because plant resources continue to play a central role in the livelihood of the rural poor in Africa.
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