Abstract
Multi resistance to antibiotics is a serious and disseminated clinical problem, common to several new compounds that block the resistance mechanism. The present study aimed at the comparative study of silver nanoparticles synthesized through actinomycetes and their antimicrobial metabolites with standard antibiotic. Marine actinomycetes collected from Gulf mannar costal region, Kayalpatnam, located at Tuticorin district, Tamil nadu, India. Totally, five actinomycetes were isolated and identified based on their spore formation and biochemical studies. Three isolates belonged to the genera of Streptomyces sp and two were Micromonospora sp. Streptomyces sp KPMS3 showed potent antibacterial activity against Gram negative pathogens. Gram positive isolates are sensitive to Micromonospora sp (KPMM2). Among the five isolates, isolate Micromonospora sp (KPMM2) was found to be an effective silver nanoparticle synthesizer. The obtained silver nanoparticles were characterized using UV-Vis spectroscopy, FTIR and TEM. The morphology of nanoparticle is found to be spherical and an average size of ranges between 38 to 52 nm. The antimicrobial activities of silver ion against test pathogens were found to be superior to cephalosporin antibiotic. The in vitro hemolytic assessment of silver nanoparticles were found to be non-hemolytic at maximum of 20 µg/ml. It was found that smaller silver nanoparticles synthesized by microbial route had a greater antibacterial activity and less hemolytic in nature. Key words: ESBL, cefotaxime, drug resistant and hemolysis.
Highlights
Hospital acquired infection are a cause of prolonged hospital stay and contagious which may lead to high morbidity and mortality throughout the hospital patients
Antibiotic resistant hospital infection can be especially deadly because antibiotics are used intensely in hospitals compared with the community and frequent use drives the development of multi drug resistance bacteria
The present work was used to carry out the synthesis of silver nanoparticle by marine actionomycets collected from Gulf Mannar Costal Region, Kayalpatnam, located at Tuticorin district, Tamil nadu, India
Summary
Hospital acquired infection are a cause of prolonged hospital stay and contagious which may lead to high morbidity and mortality throughout the hospital patients. In India, 10 to 30% patients are admitted in hospitals or nursing homes are associated with hospital acquired infection. The failure of this infection due to the development of drug resistance organisms and the rate is 3.4% (Khoiee et al, 2008). Microbes are known to fit on inanimate source such as touch surface for extending period of time. This can be making troublesome in hospital environments where patients with weakened immunity are at increased risk for contracting hospital acquired infection (Hidron et al, 2008). Over a past several years, silver nano particle are synthesized by a potential new antibiotics developer actinomycetes from marine sediments (Absar et al, 2003)
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