Abstract
Gallic acid (GA) is a functional ingredient abundant in Chinese pu-erh tea. The aim of this study was to increase the GA content in green tea extracts using acid hydrolysis. (-)-Epigallocatechin gallate treated with 1 M hydrochloric acid at 110°C for 1 h resulted in a GA yield of 45.6%. However, under these conditions, (-)-epigallocatechin was easily oxidized and rendered undetectable. On applying the same treatment to green tea extracts of Korea-cultivated Chamnok, a native species, and Yabukita, the GA contents increased from 0.17 to 4.87, 0.28 to 5.33 and 0.17 to 4.44 mM, respectively. In Chamnok extracts prepared following harvesting at three different time points, the GA contents increased from 0.17 to 4.48, 0.12 to 5.16 and 0.06 to 5.71 mM. Therefore, it is possible to produce green tea extracts with high GA concentrations using simple acid hydrolysis. This will greatly benefit the production of functional ingredients and will be useful in the beverage industry. Key words: Domestic cultivar, EGCG, EGC, tannase.
Highlights
Green tea is consumed globally and has been reported to have beneficial effects on fat oxidation and energy expenditure; it favors weight loss (Kim et al, 2020)
The epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG), or Gallic acid (GA) contents varied depending on the Korean domestic cultivar
Consistent with findings reported in a previous study (Wang et al, 2019), assessment of the catechin content in green tea extracts from various cultivars revealed that EGCG was the most abundant, followed by (-) -epigallocatechin (EGC), ECG, EC, and C
Summary
Green tea is consumed globally and has been reported to have beneficial effects on fat oxidation and energy expenditure; it favors weight loss (Kim et al, 2020). Green tea contains catechins, which represent a major. The health benefits of green tea have been highly attributed to these polyphenolic compounds (Shang et al, 2020). Green tea catechins, especially EGCG, reduce the incidence of cancers, demonstrate antiviral activity, and reportedly ameliorate collagen-induced arthritis, oxidative stress-induced neurodegenerative diseases, and streptozotocin-induced diabetes (An et al, 2020; Hayakawa et al, 2020; Izzo et al, 2020; Mohan et al, 2020). EGCG tends to reduce body weight and fat
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