Abstract
Prunus africana (Kanda stick) is a valuable medicinal tree classified as priority species for domestication in Cameroon. To measure the ability for developing axillary buds and multiple shoots in relation with the origin and state of explants, some biochemical parameters were assessed in eight types of single-node cuttings. These cuttings were treated with different concentrations of kinetin (kin) indole butyric acid (IBA) and thidiazuron (TDZ) and cultured in Murashige and Skoog (MS) medium. The glutelin content (1.13 ± 0.201 µg/mg FW) was higher compared to those of total albumin, globulin and prolamin and most representative at mature vegetative apical stage (MVA). The highest (15.60 ± 1.966 µg/mg FW) and lower (2.82 ± 0.072 µg/mg FW) amount of phenol compound was observed in the mature dormant stages and immature vegetative distal stage (IVD), respectively. Regarding the peroxidases, highest activity (0.453 ± 0.055 OD/mg/ FW.5 min -1 ) was obtained in mature dormant apical stage. Apart from IVA state, there was no reactive explant cultured in vitro. The high percentage of budding and proliferation of budding (100%) and rooting (100%) were achieved with 12 μM Kin and 24 μM IBA, respectively. In the presence of 24 μM TDZ, multiple shoot was induced with a maximum of 5 shoots/explants. Micropropagation success of P. africana is influenced by cytokinin, physiological and biochemical status of single node cutting. Key words : Biochemical profile, Prunus africana, budding, multiple shoots, rooting.
Highlights
Prunus africana (Hook.f.) Kalman commonly known as Pygeum, is a large tree which grows in the afromontane forests between 1500 and 3000 m asl (Betti and Ambara, 2013)
The biochemical studies were conducted on cuttings at different stages during growth process; mature cuttings at Dormant stage (MDA, MDD); Mature cuttings at Vegetative stage (MVA, MVD); Immature cuttings at Dormant stage (IDA, IDD); Immature cuttings at Vegetative stage (IVA, immature vegetative distal stage (IVD))
Results reveal that all the reserve proteins were represented in each developmental stage but with a differential expression
Summary
Prunus africana (Hook.f.) Kalman commonly known as Pygeum, is a large tree which grows in the afromontane forests between 1500 and 3000 m asl (Betti and Ambara, 2013). 2002) and one of the main sources of supply to satisfy this demand consists of Cameroon natural populations (Nsawir and Ingram, 2007) Much of this exploitation has been irresponsible with entire trees being girdled of their bark and left to die or, in other cases, felled to facilitate easier access to their bark (Betti, 2008). This massive exploitation leads to classify it as an endangered species in Cameroon (Cunningham, 2005). The key challenge is to develop different methods of propagation that are both appropriate to the species and applicable to local communities
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
