Abstract
Ten newly developed microsatellite loci were isolated from an AG20 and CAG20 enriched genomic DNA library of Radix sp. from Iraq. The simple sequence repeats (SSR) comprised 76.0% di-, 20.0% tri- and 4.0% tetra-nucleotide repeats. The number of alleles for all 10 loci ranged from 4 to 18, with a mean of 11.3 alleles per locus. The observed (Ho) and expected heterozygosity (He) values varied from 0.286 to 0.8670.927 (mean 0.640) and from 0.263 to 0.939 (mean 0.753), respectively. The polymorphic information content (PIC) values ranged from 0.336 to 0.923 (mean 0.749). Five loci showed significant deviation from Hardy-Weinberg equilibrium (HWE) and no significant linkage disequilibrium (LD) was observed. Cross transferability was successfully tested across on species from the genus Melanopsis. These polymorphic SSRs will be useful for assessment of the genetic diversity and population genetics of a Radix sp. as well as other related species in Iraq. Key words: Cross-transferability, eenriched genomic DNA library, ffreshwater snail, microsatellitemicrosatellite.
Highlights
The genus Radix Montfort, 1810 is a genus of freshwater snails belonging to the family of Lymnaeidae (Gastropoda: Pulmonata) which consists of 1800 species and 34 genera (Hubendick, 1951; Vinarski, 2013)
While enrichment increased the number of positive clones which contained microsatellite motifs, the level of clone redundancy was still quite high, albeit similar to the redundancy rate found in the study of Zane et al (2002)
The polymorphism rate of simple sequence repeats (SSR) primers developed from Radix sp. enriched library was slightly lower than other species of freshwater snail genus such as Physa acuta (Monsutti and Perrin, 1999), Aplexa marmorata (Dubois et al, 2008) and Bellamya aeruginosa (Gu et al, 2015)
Summary
The genus Radix Montfort, 1810 is a genus of freshwater snails belonging to the family of Lymnaeidae (Gastropoda: Pulmonata) which consists of 1800 species and 34 genera (Hubendick, 1951; Vinarski, 2013). Species identification of this family is classically based on morphological characteristics of the shell (Jackiewicz, 2000), reproductive organs (Hubendick, 1951) and mantle pigmentation (Jackiewicz, 1998), more recently, identification has included molecular characterization (Pfenninger et al, 2006; Dung et al, 2013).
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