Abstract
Tissue fixation with 10% formalin and molten paraffin wax embedding (FFPE) is routinely used protocol for tissue preservation in histopathology laboratory. We therefore aimed at comparing the differences in DNA quantity, DNA purity, nucleic acid and its effect on primers (PCR) amplicon (bp) sizes between fresh cervical tissues and formalin fixed paraffin embedded (FFPE). The differences in DNA purity, quantity and nucleic acid were 2.02±0.42 versus 1.34±0.28, 47.73±37.45 vs. 21.84±25.52 (ng/µl) and 1.56±0.59 vs 0.49±0.46 between for fresh cervical tissue and FFPE respectively and were all statistically significant at p<0.005. The difference in amplification successes was higher for the 120 bp than for the 450 bp primers. The distribution of cervical human papillomavirus for fresh tissues and formalin-fixed paraffin-embedded tissues studied was 39% and 13% respectively. Although, the present results showed that PCR genomic DNA can be extracted from both fresh cervical smear and 8 years duration FFPE archived tissue blocks. Formalin fixed paraffin embedded tissue blocks is not recommended for epidemiological study for detection and typing of cervical human papillomavirus using high molecular weight base pair primers and conventional PCR. Key words: Amplicon size, polymerase chain reaction (PCR), cervical tissues.  
Highlights
Tissue fixation with 10% Formalin and molten paraffin wax embedding is a time saving and less expensive technique. It is the most widely adopted method of preserving human tissue biopsy and biological specimens. This has resulted in increased amount of formalin-fixed paraffin-embedded tissue blocks that represent both the pathology and pre-analytical handling of biological specimens
This reservoir of these specimens is increasingly being used for DNA, RNA, and proteomic studies
A total of one hundred cervical samples were analysed, comprising of fifty fresh cervical smears and fifty formalin fixed paraffin embedded cervical tissue blocks retrieved from the pathology laboratory
Summary
Tissue fixation with 10% Formalin and molten paraffin wax embedding is a time saving and less expensive technique. It is the most widely adopted method of preserving human tissue biopsy and biological specimens. This has resulted in increased amount of formalin-fixed paraffin-embedded tissue blocks that represent both the pathology and pre-analytical handling of biological specimens. This reservoir of these specimens is increasingly being used for DNA, RNA, and proteomic studies.
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